Kinetic Studies of Adenosine Kinase from Ehrlich Ascites Tumor Cells

Abstract

Abstract Adenosine kinase (ATP: adenosine 5'-phosphotransferase, EC 2.7.1.20) was partially purified from Ehrlich ascities tumor cells. Initial velocity and product inhibition studies indicated that it catalyzes an ordered Bi Bi reaction, with ATP the first substrate to bind, and ADP the first product to dissociate from, the enzyme.