CELL WALLS OF PIRIGULARIA ORYZAE

Abstract

Cell walls were prepared from Piricularia oryzae P2. Lytic enzymes were produced when Bacillus circulans WL 12 was grown with P. oryzae cell wall as a sole source of carbon. Mode of lysis of P. oryzae cell walls was compared with the lysis of walls of Aspergillus oryzae and Neurospora crassa. Activities of β-1→3-glucanase, chitinase, and β-1→6-glucanase were demonstrated in the culture filtrate. Modes of lysis of cell walls of P. oryzae P2 by a single and combined action of lytic enzymes were compared. Combination of β-1→3-glucanase and chitinase gave the most rapid and extensive lysis.