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Effect of neocarzinostatin on DNA synthesis in L1210 cells.
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1974
Year
Dna Polymerase ReactionBiosynthesisCell RegulationBiochemistryNatural SciencesMedicineDna SynthesisBiotechnologyDna ReplicationMolecular BiologyEnzymatic ModificationPharmacologyCell BiologyL1210 Cells
The mechanism of action of neocarzinostatin has been investigated. The synthesis of DNA in L1210 cells in vivo was selectively inhibited by the drug. DNA synthesis in vitro catalyzed by crude DNA-dependent DNA polymerase of L1210 cells was sensitive to the drug in a concentration-dependent manner. This inhibition was reversed by increasing the concentration of DNA. Evidence for a DNA-drug interaction was obtained from the results of the preincubation of DNA with the component in the DNA polymerase reaction and from thermal denaturation studies. The degradation of cellular DNA in vivo by the drug was also suggested by the sedimentation profile in the alkaline sucrose gradient centrifugation.