Influence of Kynurenate on Cholesterol and Fatty Acid Synthesis in Isolated Perfused Rat Liver

Abstract

Abstract Cholesterol and fatty acid synthesis of the isolated perfused rat liver were measured by the 3H2O method. Kynurenate (10 to 40 mm) inhibited the rate of fatty acid synthesis by 60 to 88%. The rate of cholesterol synthesis, on the contrary, was not reduced at all by this inhibitor. These findings show that malonyl coenzyme A is not an intermediate in β-hydroxy-β-methylglutaryl-CoA formation in the intact organ and exclude the existence of a malonyl-CoA pathway of cholesterol synthesis in rat liver.