THE DISTRIBUTION OF ELECTROLYTES IN DOGS FOLLOWING LIGATION OF BOTH URETERS

Abstract

Distribution of Electrolytes in Dogssame time samples of skin and muscle were taken from Dogs VI, I-111, X, and XIII; and from Dogs X and XIII, a sample of brain tissue through a small trephined opening in the skull.After operation the dogs were placed in a metabolism cage, receiving no food and varying amounts of water (see Table III).In about 3 days the animals were very sick and were bled again.At autopsy, further samples of skin, muscle, and brain were obtained.In Dog IT' the contents of the stomach and intestines were removed and analyzed for chloride and total base.-1 100 cc.Luer syringe containing 10 cc. of albolene was used for removing the 55 cc. of blood required for the examinations.3 cc.were oxalated for hemoglobin, hematocrit, and whole blood phosphate determinations; 6 cc.were run under oil into an oxalate tube for whole blood COZ content and whole blood chloride; 2 cc.were run into two flasks for whole blood total base (see below); and t,he remainder was placed under oil in a narrow necked 50 cc.centrifuge tube, which was then covered with a rubber cap.This last sample was allowed to clot, centrifuged, and a sample removed for estimation of serum COZ content.The rest of the serum was pipetted off and used for phosphate, chloride, non-protein nitrogen, and, in some instances, sulfate determinations.The hemoglobin determination was done on a modified Sahli appara,tus (glass rod standards) st'andardized by oxygen capacity determinations.Hematocrits were done in duplicate, centrifuged to constant reading.It was found to be rather difficult to obtain satisfactory results from blood mixed with oil, so, with probably insignificant sacrifice of accuracy, we did not use samples oxalated under oil.For whole blood total base, 8 cc. of distilled water were put in a 10 cc.volumetric flask and blood run in from the syringe up to the mark; the flask was then shaken until hemolysis was complete, when a 5 cc.aliquot was quickly removed for total base determination.The method employed is a modification of the Fiske method for total base in urine (1).The same method was applied to serum and to vomitus.Chlorides in whole blood, serum, vomitus, and tissues were determined according to Van Slyke's method (2); CO2 content by the method of Van Slyke and Neil1 (3).Total nitrogen was estimated by Kjeldahl (macro), non-protein nitrogen by Folin's