Inactivating and Mutagenic Effects of 1-Methyl-3-Nitro-1-Nitrosoguanidine on Intracellular Bacteriophage

Abstract

1-Methyl-3-nitro-1-nitrosoguanidine (NG) was able to inactivate and mutate adsorbed but not free coliphage T2. Coliphage T4 was as sensitive to NG as T2. When treatment was delayed for 5 min or longer after adsorption of T2, the phage became increasingly refractory to NG inactivation.