Inhibition of cellular proliferation and enhancement of hydrogen peroxide production in fibrosarcoma cell line by weak radio frequency magnetic fields

TLDR

The study investigates how weak radio‑frequency magnetic fields affect hydrogen peroxide production and proliferation of fibrosarcoma HT1080 cells in vitro. Cells were exposed to 45 µT static magnetic fields oriented vertically to the growth plane, alone or combined with 5 or 10 MHz RF fields of 10 µT RMS intensity perpendicular to the static field. Exposure to a 10 MHz RF field with the static field reduced cell numbers by up to 30 % on day 2 and increased hydrogen peroxide production by 55 %, indicating that magnetic fields elevate H₂O₂ levels while suppressing cell growth. Bioelectromagnetics 35:598–602, 2014; © 2014 Wiley Periodicals, Inc.

Abstract

This study presents experimental data for the effects of weak radio frequency (RF) magnetic fields on hydrogen peroxide (H 2 O 2 ) production and cellular growth rates of fibrosarcoma HT1080 cells in vitro. Cells were exposed either to 45 µT static magnetic fields (SMFs)‐oriented vertical to the plane of growth or to SMFs combined with weak 5 and 10 MHz RF magnetic fields of 10 µT RMS intensity perpendicular to the static field. Cell numbers were reduced up to 30% on Day 2 for the cells exposed to the combination of SMF and a 10 MHz RF magnetic field compared with the SMF control cells. In addition, cells exposed to 10 MHz RF magnetic fields for 8 h increased H 2 O 2 production by 55%. The results demonstrate an overall magnetic field‐induced biological effect that shows elevated H 2 O 2 levels with accompanying decrease in cellular growth rates. Bioelectromagnetics 35:598–602, 2014. © 2014 Wiley Periodicals, Inc.