Computer Control of Microscopes Using µManager

TLDR

Computer‑controlled microscopes, enabled by digital cameras and motorized components, are increasingly important, and acquisition software must be user‑friendly while fostering innovative methods. The open‑source software package µManager aims to fulfill those goals. The unit offers step‑by‑step protocols for beginning and advanced use of µManager. Curr.

Abstract

Abstract With the advent of digital cameras and motorization of mechanical components, computer control of microscopes has become increasingly important. Software for microscope image acquisition should not only be easy to use, but also enable and encourage novel approaches. The open‐source software package µManager aims to fulfill those goals. This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software. Curr. Protoc. Mol. Biol . 92:14.20.1‐14.20.17. © 2010 by John Wiley & Sons, Inc.