A Guanosine Triphosphate-dependent Acyl Coenzyme A Synthetase from Rat Liver Mitochondria

Abstract

A guanosine triphosphate-specific acyl coenzyme A synthetase has been purified from extracts of sonically disrupted rat liver mitochondria. This enzyme, which is free of adenosine triphosphate-dependent activating systems and from succinyl-CoA synthetase activity, catalyzes the formation of CoA esters of both long and short chain fatty acids. GTP-specific fatty acid activation is inhibited by orthophosphate and fluoride.