Properties of the bound coenzyme and subunit structure of omega-amino acid:pyruvate aminotransferase.

Abstract

w-Amino acid:pyruvate aminotransferase of Pseudomonas sp.F-126 is composed of 1600 amino acids/ mol of enzyme, including 8 residues each of half-cystine and methionine/subunit.The enzyme was shown to consist of four subunits identical in molecular weight by sodium dodecyl sulfate-disc electrophoresis.The identity of the subunits is further suggested by determining NH2-and COOH-terminal amino acid sequences.The NHz-terminal amino acid sequence is Asn-Met-Pro-Glu-His-Ala-Gly-Ala-Ser-Leu-Ala-Ser-Gln-Leu-Lys-Leu-Glu-Ala.The sequence of Leu-Asp is shown for COOH terminus of the enzyme with carboxypeptidase A and Y digestions.The enzyme exhibits absorption