Amino acid composition of fungi during development in submerged culture

Abstract

Methods of culture Conidia from 10-to 20-day cultures on Czapek-Dox agar slopes were suspended in a sterile 0 05 % solution of an anionic detergent (Teepol; Shell Chemical Co.), centrifuged and resuspended in sterile water at 0-8 x 10-7-12 x 107 conidia/ml. (by haemocytometer count). Then 10 ml. volumes of conidial suspension were transferred to 700 ml. round flasks, each containing 200 ml. of medium. Flasks were plugged with cotton-wool and incubated at 250 on a reciprocating shaker (240 strokes/min.). The fungi grew as well-dispersed suspensions of hyphae; individual thalli were separate or in loose tangles.