Nucleotide Sequence of 4.5 S Ribonucleic Acid of Novikoff Hepatoma Cell Nuclei

Abstract

Abstract The primary sequence of nucleotides has been defined for 4.5 S RNAi, which is one type of low molecular weight nuclear RNA of Novikoff hepatoma ascites cells. This RNA is the first unique nuclear species of mammalian RNA to be sequenced. It is specifically localized to the extranucleolar portion of the nucleus and is not a precursor of tRNA. Highly labeled 4.5 S RNAi was obtained from cells incubated with [32P]orthophosphate in vitro. The RNA was purified by successive gel electrophoresis and chromatography on DEAE-Sephadex. The primary sequence of nucleotides of this RNA shows that it has a purine-rich 5' end and a pyrimidine-rich 3' end: [see PDF for sequence].