Reconstitution of a functional coupling factor from the isolated subunits of Escherichia coli F1 ATPase.

Abstract

The a, 8, and y subunits of Escherichia coli coupling factor ATPase (ECF1) were isolated by a method described previously (Futai, M. ( 1977) Biochem Biophys.Res.Cornmum 79, 1231-1237) and by an improved method using hydroxyapatite and DEAE-Sepharose chromatography.The isolated a subunit bound ATP and ADP (1 mol of nucleotide/mol of a) with KO values