PubMed · 1975 · 458 citations · 0 references
ImmunologyPathologyTumor CellsEfferent BloodCancer BiologyTumor BiologyArterial BloodCancer ResearchHistopathologyMammary GlandEfferent Tumor BloodMalignant DiseaseCell BiologyTumor MicroenvironmentEndocrine-related CancerBreast CancerMammary Gland BiologyMedicineCancer Growth
The study uses a hormone‑dependent MTW9 rat mammary carcinoma model in which tumor growth is induced by reducing host mammotropin levels. Tumor cell shedding into efferent tumor blood was quantified, and tumor cells were distinguished from leukocytes by indirect immunofluorescence. Growing and regressing tumors shed similar numbers of cells (~3–4 × 10⁻⁶ cells g⁻¹ 24 h), with arterial blood containing 12‑fold fewer cells, showing that shedding plays a minor role in cell loss, is not enhanced during regression, and that circulating tumor cells are rapidly cleared yet sufficient to seed new tumors.
The rate of tumor cell shedding into efferent tumor blood was measured in growing and regressing MTW9 rat mammary carcinomas. The hormone-dependent tumor, grown as an 'isoession was induced by reduction of mammotropin level in the host. Tumor cells were differentiated from normal leukocytes by indirect immunofluorescence. Growing tumors shed 3.2 x 10-6 and regressing tumors shed 4.1 x 10-6 cells per 24 hr per g tissue. Cell shedding rates of growing versus regressing tumors were not siginificantly different over a tumor size range of 2 to 4 g. The number of tumor cells in the arterial blood was 12-fold smaller than in the efferent tumor blood. It is concluded that: (ay cell shedding via blod probably plays only a minor role in the total cell loss by gtowing MTW9 carinomas; (b) hormone-induced tumor regression does not depend on increased cell shedding; (c) tumor cells are rapidly cleared from circulating blood; and (d) a 2-g MTW9 carcinoma pours enough cells into the host circulation to transplant the tumor every 24 hr.