Two Crystal Forms of Bovine Superoxide Dismutase

Abstract

Abstract In the course of x-ray crystallographic investigation of superoxide dismutase, two suitable crystal forms of the bovine enzyme have been obtained. A monoclinic form grown from 58% 2-methyl-2,4-pentanediol has space group C2 and the following cell dimensions: a = 93.4 A, b = 90.4 A, c = 71.6 A, β = 95.1°, with 4 subunits (2 molecules) per asymmetric unit. An orthorhombic form grown from concentrated protein solution at 4° has space group P212121, and a = 51 A, b = 61 A, and c = 147 A, probably with 4 subunits per asymmetric unit.