Concepedia

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

Robert Sikorski, Jef D. Boeke

Methods in enzymology on CD-ROM/Methods in enzymology · 1991 · 589 citations · 25 references

DOI

Concepts

References

25

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text

, , +17

Transformation of intact yeast cells treated with alkali cations

Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983 · 7.7K citations · Full text

, , +10

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink · Molecular and General Genetics MGG · 1984 · 2.4K citations

, , +8

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

James R. Broach, Jeffrey N. Strathern, James Hicks · Gene · 1979 · 1.1K citations

, , +11

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

Mark J. Zoller, Michael J. Smith · DNA · 1984 · 906 citations

, , +15