Plant Pathology · 2010 · 68 citations · 32 references
Life SciencesPlant BiologyPlant SciencesMycologyPhylogeneticsPlant GeneticsBacterial Plant PathogensEpidemiology Of Plant DiseasesPathogen IsolationPlant PhylogenyPlant-pathogen InteractionsPlant-pathogenic FungiFusarium Root RotMating Type SystemsMating-type LociMolecular Phylogenetics Of FungiPhylogenetic Character AnalysisPhylogenetic Diversity AnalysisPhylogenetic ReconstructionPolymerase Chain Reaction (Pcr) Amplification
Isolates of Fusarium spp. were recovered from the roots of field pea ( Pisum sativum ) collected from 15 commercial fields in Alberta, Canada. Most of the isolates (75 out of 96) were identified as F. avenaceum , based on morphology, phylogeny and species‐specific PCR amplification. Molecular differences in the F. avenaceum isolates were detected based on putative mating type, and on ITS and CPN60 sequences. MAT‐1 and MAT‐2 were equally distributed among the isolates. Phylogenetic analysis based on ITS and CPN60 sequences clustered most of the F. avenaceum isolates into a single group. In some cases, isolates with low aggressiveness clustered together in additional groups. There was no correlation between phylogenetic profile and either mating type or geographic origin. This population of F. avenaceum has a low level of genetic variation and consists of isolates derived from the two mating types. Isolates with low aggressiveness are also retained in the population.
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PCR Protocols-A Guide to Methods and Applications
Sarah J. Gurr · Biochemical Education · 1991 · 1.2K citations
Kerry O’Donnell, Todd J. Ward, David M. Geiser et al. · Fungal Genetics and Biology · 2004 · 767 citations
Rapid Mini-Preparation of Fungal DNA for PCR
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