ON THE DISTRIBUTION OF THE NON-PROTEIN SULFUR OF THE BLOOD BETWEEN SERUM AND CORPUSCLES

Abstract

The work in this paper presents the results obtained in a series of analyses made with the idea of obtaining data on the distribution of the non-protein sulfur of the blood between corpuscles and plasma.Our determinations were made on blood in which coagulation had been prevented by the addition of 5 gm. of powdered sodium citrate per liter.We are aware of the fact that the use of an anticoagulant of the nature of sodium citrate is undesirable in an investigation of this type; but as the conditions under which we were able to obtain our samples of human blood made the use of this substance necessary, we felt it desirable, for purposes of comparison, to collect our specimens of animal blood in the same way.The analytical methods used were those recently described by Denis and Reed."Isl every case the blood proteins were precipitated as soon as possible after the collection of the blood samples, although in some cases more than an hour elapsed before this procedure could be carried out due to the time required to transport the specimens from the slaughter-house or hospital.The results obtained on the blood and plasma of the dog, beef, and goat are presented in Table I while those on human blood are given in Table II.In the case of the three species of animals investigated the inorganic and ethereal sulfate fractions appear to be distributed 1