HPLC Practical and Industrial Applications

Abstract

INTRODUCTION, Jeffrey R. Larson, James E. Tingstad, and Joel K. Swadesh Overview Pumps Columns Chromatographic modes Detectors Chromatographic theory Laboratory operations The role of laboratory management AUTOMATED SAMPLING IN THE PROCESS ENVIRONMENT, Jeffrey R. Larson Issues in process HPLC Case histories Future directions for on-line HPLC process monitors Summary CHROMATOGRAPHY IN PROCESS DEVELOPMENT, Patricia Puma Scope of process chromatography Special hardware requirements Validation considerations Case history Summary REVERSED PHASE HPLC, Rekha D. Shah and Cynthia A. Maryanoff Introduction Chromatographic practice in RPLC Selected applications Case history: optimizing a chemical synthesis by RPLC Conclusions ION EXCHANGE CHROMATOGRAPHY, Joel K. Swadesh Introduction Ion exchange stationary phases Electrochemical detectors Applications Case history: isolation and partial characterization of hementin, a compound of biopharmaceutical interest Acknowledgments GEL PERMEATION CHROMATOGRAPHY, Rajesh G. Beri, Laurel S. Hacche, and Carl F. Martin Introduction Detectors for gel permeation chromatography Application of aqueous size-exclusion chromatography352 to carbohydrates: a case history HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS: AN OVERVIEW, Kalman Benedik and Andras Guttman Introduction Fundamentals Instrument Separation modes Applications Conclusions Index