Effects of pH treatments and deglycosylation of rabbit immunoglobulin G on the binding of C1q.

Abstract

The abbreviations used are: Ig, immunoglobulin; Asn-GlcNAc, the N-glycosidic linked pair 1-L-0-aspartamido-2-acetamido-2-deoxy- /I-D-glucose found as the core-type linkage between the polypeptide backbone and oligosaccharides of certain glycoproteins; (BADL-pro)?-EDA, bis(a-bromoacetyl-~-2,4-dinitrophenyl proly1)ethylenediamine; BDPE, bis-2,4-dinitropheny1 pimelic ester; C' , complement; CF-saline, 0.15 M