Specificity of Radioiodinated (I131) Immune Globulin As Determined by Quantitative Precipitin Reaction

Abstract

Summary The reaction between human serum albumin and I131 trace iodinated rabbit antibody was studied by the quantitative precipitin reaction. The amount of radioactivity found in each immune precipitate was proportional to the antibody nitrogen precipitated. Under the iodinating conditions described (1.3 iodine atoms per antibody molecule) no loss of immunologic specificity of rabbit antibody labelled with I131 was demonstrated.