Inhibition of N-linked complex oligosaccharide formation by 1-deoxynojirimycin, an inhibitor of processing glucosidases.

Abstract

H3G I Y6Glucosidase activities which remove glucose residues from GlcZMangGlcNAcz and GlclManyGlcNAcz oligosaccharides were obtained in soluble form from Saccharomyces cerevisiae X-2180 without detergent.These two enzyme activities were clearly separated from the GlcsMangGlcNAcz oligosaccharide glucosidase which was shown previously to remove the terminal glucose residue from GlcsMan9GlcNAcz oligosaccharide (Kilker,