Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses.

Abstract

Summary A cell line (JLS-V9) established from the bone marrow of normal weanling BALB/c mice has been shown to be susceptible to infection with either the Rauscher or Moloney murine leukemia viruses. The Rauscher- and Moloney-virus-infected cultures were designated JLS-V10 and JLS-V11, respectively. Both infected cultures were carried for 7 months without loss of infectivity. However, more recent bioassays of Rauscher virus from the JLS-V10 line have indicated a reduction of infectivity for BALB/c mice. Virus multiplication was evaluated both by electron microscopy and bioassays of cell free tissue culture fluids. The viruses were identified by serum neutralization and response of infected mice to the respective viruses, as well as electron microscopy. Cytopathogenic effects of the viruses have not been observed in either of the infected cultures.