Patterns of three-dimensional growth in vitro in collagen-coated cellulose sponge: carcinomas and embryonic tissues.

Abstract

Summary Several transplantable tumors and chick embryonic tissues were cultured in collagen-coated cellulose sponge. The growth obtained in this matrix was compared with that obtained in cellulose sponge alone and in collagen sponge alone. The tumors included a mouse mammary adenocarcinoma (C3HBA), a squamous cell carcinoma of the mouse cervix (D-98), a mouse ascites hepatoma (Sato A H #134), a rat ascites hepatoma (Yoshida #7974), and a human adenocarcinoma of the colon (H.Ad.-1). The embryonic tissues were intestine, lung, and heart. All the sponges were examined in histologic section. The best patterns of migratory outgrowth were seen in collagen-coated cellulose sponge. Conventional histologic criteria were used to identify the cellular components of the newly formed tissues.