Application of o-hydroxyhydroquinonephthalein-iron(III) complex to determination of organic compounds containing phosphorus.

Abstract

An application of o-hydroxyhydroquinonephthalein-iron(III) complex to the determination of oranic compounds containing phosphorus, such as adenosine 5'-triphosphate (ATP), was developed. In the determination of ATP, Beer's law held up to 4μg of ATP in the final volume of 10ml, the apparent molar absorptivity being 1.1×106dm3mol-1Cm-1. Other compounds containing phosphorus such as glucose-6-phosphate, phytic acid and L-α-lecithin could also be determined under the standard conditions. The proposed method, in which the use of buffer and surfactant solutions was modified, is relatively simple, does not require preliminary treatment and is about 5 times more sensitive than previous method.