Tracer studies on the metabolism of the Gardner lymphosarcoma. I. The uptake of radioactive glycine into tumor protein.

Abstract

The incorporation of glycine-2-C14 into the protein of the Gardner lymphosarcoma cells has been studied. The process is not markedly affected by changes in the Ca++, Mg++, or K+ ion concentrations of the medium or by changes in the hydrogen ion concentration between pH 7 and 8. Below pH 6.5, the uptake is inhibited. Glycine uptake is stimulated by glucose and by bicarbonate and, in the presence of these, is linear for 2 hours. Metabolic inhibitors or conditions whereby the cells are disrupted almost completely abolished uptake.