Phenolic constituents of agrimony [Agrimonia eupatoria L.] herb

Abstract

The total content of tannins (10.08%), flavonoids (0.33%) and phenolic acids (2.26%) in agrimony herb collected from natural sites in eastern Poland were determined. On the basis of GC, twelve phenolic acids were identified, with p-hydroxybenzoic acid as a dominant compound, present mainly in a bounded form. Protocatechuic and vanillic acids were the main components of the fraction of free phenolic acids. Five flavonoid compounds, not reported in the herb ofA. eupatoria before, i.e. luteolin 7-O-sophoroside, luteolin 7-O (6- acetylglucoside), acacetin 7-O-glucoside, luteolin 7-O-glucoside and apigenin 7-O-glucoside were isolated and their structure elucidated by chemical and spectral methods (UV, 1 H NMR, 1 3 C NMR).