Epinephrine and Adrenocorticotropic Hormone-stimulated Magnesium Accumulation in Adipocytes and Their Plasma Membranes

Abstract

Epinephrine, norepinephrine, and adrenocorticotropic hormone stimulate magnesium accumulation in plasma membrane vesicles derived from fat cells.A new method was developed to demonstrate this magnesium uptake utilizing the ligand, 8-hydroxyquinoline-5-sulfonic acid, whose magnesium complex fluoresces distinctively.An increase in total magnesium content in whole fat cells following hormone stimulation was documented.These findings were confirmed with 28Mg2+ in intact adipocytes.