Effect of interleukin-1 on intracellular concentration of sodium, calcium, and potassium in 70Z/3 cells.

Abstract

The cytokine interleukin-1 (IL-1) has been shown to induce the differentiation of a murine pre-B like cell line 70Z/3. An early intracellular event caused by exposure to IL-1 is an amiloride-sensitive progressive rise in the total concentration of intracellular sodium ([Na]i), caused by influx of Na+ from outside, and a transient fall in total intracellular calcium. The results suggest that IL-1-induced influx of Na+ into 70Z/3 occurs via stimulation of the Na+/H+ antiport. IL-1-induced differentiation is also blocked by amiloride suggesting that stimulation of Na+/H+ exchange may play a role in IL-1-induced differentiation.