Human Carbonic Anhydrases

Abstract

Abstract A combination of hydroxylapatite and diethylaminoethyl cellulose chromatographic fractionation procedures have been used to isolate a relatively large number of human erythrocyte carbonic anhydrases. Some of the crystalline minor isozymes isolated show two starch gel electrophoretic components, closely related in mobility. A combination of immunological, enzymatic, and 65Zn exchange reactions, in conjunction with starch gel electrophoretic separations, has permitted the demonstration of the presence in hemolysates of carbonic anhydrase isozymes with the same electrophoretic properties as the isolated isozymes.