Isolation and partial characterization of multiple DNA polymerases of the murine myeloma, MOPC-21.

Abstract

A method is presented for the isolation and initial purification of the DNA polymerases of the myeloma line MOPC-21. These activities are separable into two distinct fractions by diethylaminoethyl cellulose chromatography. Each fraction contains a DNA polymerase that is highly active on native calf thymus DNA that had been partially degraded with DNase (“activated” or “nicked” DNA) and another activity that transcribes ribopolymer strands in the presence of a complementary primer. Some distinctive properties of these enzymes are presented.