Effects of insulin and EGF on DNA synthesis in bovine endothelial cultures: flow cytometric analysis.

Abstract

Flow cytometry was a useful technique in separating cultured bovine corneal endothelial cells according to their DNA content. Analysis of the cultures after the addition of insulin and EGF showed an increase in DNA synthesis. The synergistic effects of the growth factors on corneal endothelial cells suggest that they stimulate mitotic activity by different mechanisms. The addition of mitogens to eye bank storage media may increase corneal endothelial cell densities in donor corneas.