Physical characterization of the transferrin receptor in human placentae.

Abstract

The physical properties and binding characteristics of the solubilized transferrin receptor isolated from the placental brush-border membrane of a human trophoblast cell were investigated.The receptor protein was isolated from solubilized 1251-labeled membranes by immunoprecipitation with anti-human transferrin in the presence of saturating amounts of human transferrin.Gel filtration on acrylamide agarose (AcA-22) at 23 "C in the absence of transferrin indicates the transferrin receptor has a Stokes radius of 4.6 nm.In the presence of transferrin, the Stokes radius of the receptor shifts to 6.3 nm.Sucrose density centrifugation studies indicate that it has a sedimentation coefficient of 9.8 S in the absence of transferrin and 11.2 S in the presence of transferrin.The molecular weight for the transferrin free receptor is calculated to be 213,000.Upon incubation with transferrin, it increases to 364,000.This is consistent with the idea that the active form of the solubilized receptor is a dimer and the dimer is in turn capable of binding two transferrin molecules.* The abbreviation used is: SDS, sodium dodecyl sulfate.