Mass fragmentographic determination of indole-3-acetic acid in callus tissues of Panax ginseng and Nicotiana tabacum.

Abstract

Free indole-3-acetic acid was identified and the amount of which was estimated in extracts of auxin-requiring and nonrequiring calluses of P. ginseng and N. tabacum by means of gas chromatograph-mass fragmentography (GC-MS-MF). The data well compare with those reported for other plant materials determined by conventional methods. It has been shown that biological samples less than 1 g are sufficient to give a confident result with this method. This will enable to determine rapidly the physiological levels of natural auxins and to discuss the delicate roles of these plant growth regurators.