A Precise Method for Replicating Suspension Cultures of Mammalian Cells

Abstract

A simple, readily assembled shaker-culture system for the cultivation of mammalian cells is described. No specialized glassware and equipment were used in this system, which consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed to quantitate the effects of several variables, including medium ingredients, serial transfers, and freezing and storage on two variants of the L-cell line. This system is reproducible and precise and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis.