Heterotransplantation of a hematopoietic cell line derived from a patient with multiple myeloma.

Abstract

Summary A cell line has been established from the buffy coat of a patient with multiple myeloma. This cell line, which was designated RPMI 8226, has a triploid chromosome constitution and synthesizes only λ light-chain protein. When 10 7 or 2.5 × 10 7 viable cells were inoculated s.c. in sublethally irradiated mice, all of them developed subcutaneous tumors. Nonirradiated mice developed fewer and much smaller tumors, and histological preparations of the latter tumors revealed cell degeneration. Inoculation i.p. of 10 7 viable cells resulted in milky, cell-rich ascites tumors at the site of inoculation and small intraperitoneal tumors at incidences of 50, 37, and 30%, respectively. The total number of ascites cells amounted to approximately 2 to 10 times the number of injected cells 10 days after inoculation. Chromosomal, immunological, and cloning examinations of the cultured cells derived from induced tumors and ascites fluid showed that these cells were identical to the inoculated cells.