Evidence that stimulatory factor(s) of RNA polymerase II participates in accurate transcription in a HeLa cell lysate.

Abstract

Antibody raised against S-II, a stimulatory factor of RNA polymerase II from Ehrlich ascites tumor cells, inhibited accurate transcription from adenovirus 2 major late promoter in a HeLa cell lysate. Manganese prevented accurate transcription, but it was essential for expression of the stimulatory activity of S-II. The results indicate that protein(s) cross-reacting immunologically with S-II in a HeLa cell lysate is essential for accurate transcription of truncated DNA, but the stimulatory activity of this factor(s) is not necessary for accurate transcription of truncated DNA.