Current Protocols in Cell Biology · 2003 · 28 citations · 13 references
Life SciencesCell BiologyMolecular BiologyCytoskeletonMolecular Cell BiologyMolecular ImagingCellular ImagingCellular VisualizationFluorescence ImagingFluorescence MicroscopyFungal Cell BiologyYeast BiologyCellular Fluorescence ImagingFluorescence Labeling StrategiesFluorescent Dye LabelingFluorescent LabelingFluorescent LabelsFungal Cell WallsImmunofluorescence StainingLive-cell Fluorescence Imaging
This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.
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Christopher K. Raymond, I Howald-Stevenson, Carol A. Vater et al. · Molecular Biology of the Cell · 1992 · 841 citations · Full text
Life Sciences, Biology, Cell Biology +17
[40] Immunofluorescence methods for yeast
John R. Pringle, A.E. Adams, David G. Drubin et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 1991 · 702 citations