Crystallization and preliminary X-ray diffraction study of heavy riboflavin synthase from Bacillus subtilis.

Abstract

Crystals of heavy riboflavin synthase from Bacillus subtilis have been obtained from 1.3 M sodium/potassium phosphate, pH 8.7, containing 0.35 mM 5-nitroso-6-(1'-D-ribitylamino)-2,4(1H,3H)-pyrimidinedione. X-ray photographs indicate a hexagonal unit cell with dimensions of a = b = 156.4 A; c = 298.5 A; alpha = beta = 90 degrees; gamma = 120 degrees. The space group was established as P6(3)22 with 12 general positions.