Replication of phi X174 DNA with purified enzymes. II. Multiplication of the duplex form by coupling of continuous and discontinuous synthetic pathways.

Abstract

Synthesis by purified enzymes of viral strands from supercoiled, circular duplexes (RF), the RF --single- stranded circular DNA (SS) system, has been coupled in a single reaction mixture to the synthesis of comple- mentary strands on viral strand templates, the SS-* RF system, to achieve rapid, net synthesis of RF (Arai,