ENZYMES IN ANEMIA: A STUDY OF ABNORMALITIES OF SEVERAL ENZYMES OF CARBOHYDRATE METABOLISM IN THE PLASMA AND ERYTHOCYTES IN PATIENTS WITH ANEMIA, WITH PRELIMINARY OBSERVATIONS OF BONE MARROW ENZYMES

Abstract

Article1 November 1960ENZYMES IN ANEMIA: A STUDY OF ABNORMALITIES OF SEVERAL ENZYMES OF CARBOHYDRATE METABOLISM IN THE PLASMA AND ERYTHOCYTES IN PATIENTS WITH ANEMIA, WITH PRELIMINARY OBSERVATIONS OF BONE MARROW ENZYMESPAUL HELLER, M.D., F.A.C.P., HYMAN G. WEINSTEIN, M.S., MICHAEL WEST, M.D., HYMAN J. ZIMMERMAN, M.D., F.A.C.P.PAUL HELLER, M.D., F.A.C.P.Search for more papers by this author, HYMAN G. WEINSTEIN, M.S.Search for more papers by this author, MICHAEL WEST, M.D.Search for more papers by this author, HYMAN J. ZIMMERMAN, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-53-5-898 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptSeveral published studies1, 2, 3, 4 have shown that patients with megaloblastic anemia have markedly elevated serum levels of lactic dehydrogenase. The sera of patients with sickle cell anemia were found to contain only slightly to moderately elevated levels of this enzyme. In most other forms of anemia the serum levels were within normal limits.2The possibility was considered that these elevations of the serum or plasma lactic dehydrogenase in megaloblastic anemia might reflect an abnormality peculiar to the megaloblastic cell series, consisting of an abnormally high content of lactic dehydrogenase and increased activity of the anaerobic glycolytic cycle. This...Bibliography1. ZimmermanWeinstein HJHG: Lactic dehydrogenase activity in human serum, J. Lab. and Clin. Med. 48: 607, 1956. MedlineGoogle Scholar2. ZimmermanWestHeller HJMP: Serum enzymes in disease. II. Lactic dehydrogenase and glutamic oxalacetic transaminase in anemia, Arch. Int. Med. 102: 115, 1958. CrossrefGoogle Scholar3. GordonEnari RTM: Lactic acid dehydrogenase in vitamin B12 deficiency, Acta haemat. 21: 16, 1959. CrossrefMedlineGoogle Scholar4. HellerWeinsteinWestZimmerman PHGMHJ: Glycolytic, citric acid cycle, and hexosemonophosphate shunt enzymes of plasma and erythrocytes in megaloblastic anemia, J. Lab. and Clin. Med. 55: 425, 1960. MedlineGoogle Scholar5. FinchColemanMotulskyDonohueReiff CADHAGDMRH: Erythrokinetics in pernicious anemia, Blood 11: 807, 1956. CrossrefMedlineGoogle Scholar6. SingerKingRobin KJCS: The life span of the megalocyte and the hemolytic syndrome of pernicious anemia, J. Lab. and Clin. Med. 33: 1068, 1948. MedlineGoogle Scholar7. DrabkinAustin DLJH: Spectrophotometric studies. II. Preparations from washed blood cells: nitric oxide hemoglobin and sulfhemoglobin, J. Biol. Chem. 112: 56, 1935. Google Scholar8. LowryRosebroughFarrRandall OHNJALRJ: Protein measurement with the folin phenol reagent, J. Biol. Chem. 193: 265, 1951. CrossrefMedlineGoogle Scholar9. LuhbyCoopermanTeller ALJHDN: Histidine metabolic loading test to distinguish folic acid deficiency from vitamin B12 in megaloblastic anemias, Proc. Soc. Exper. Biol. and Med. 101: 350, 1959. CrossrefMedlineGoogle Scholar10. CarterHellerSchaffnerKorn FCPGRJ: Formiminoglutamic acid excretion in patients with hepatic cirrhosis, Arch. Int. Med. in press. Google Scholar11. YakulisHellerJosephsonSinger VPAML: The demonstration of A2 hemoglobin by agar gel electrophoresis, Am. J. Clin. Path. 34: 28, 1960. CrossrefMedlineGoogle Scholar12. SmithBaker LHFA: Pyrimidine metabolism in man. III. Studies on leukocytes and erythrocytes in pernicious anemia, J. Clin. Investigation 39: 15, 1960. CrossrefMedlineGoogle Scholar13. BockWallerLöhrKarges HEHDGWO: Besonderheiten im Fermentgehalt von Megalocyten, Klin. Wchnschr. 36: 151, 1958. CrossrefMedlineGoogle Scholar14. Beck WS: Leukocyte glycolysis: an investigation of the factors controlling the rate behavior in multienzyme systems, Ann. New York Acad. 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CrossrefMedlineGoogle Scholar21. SmithSchulmanAndoStern CHIREG: Studies in Mediterranean (Cooley's) anemia. I. Clinical and hematologic aspects of splenectomy, with special reference to fetal hemoglobin synthesis, Blood 10: 582, 1955. CrossrefMedlineGoogle Scholar22. WróblewskiWróblewski FR: The clinical significance of lactic dehydrogenase activity of serous effusions, Ann. Int. Med. 48: 813, 1958. LinkGoogle Scholar23. StrandjordThomasWhite PEKELP: Studies on isocitric and lactic dehydrogenase in experimental myocardial infarction, J. Clin. Investigation 38: 2111, 1959. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chicago, Illinois*Received for publication April 29, 1960.Presented in part at the Forty-first Annual Session of the American College of Physicians, San Francisco, California, April 5, 1960.From the Research Laboratory and the Medical Service, Veterans Administration West Side Hospital, and the Departments of Medicine, University of Illinois College of Medicine and the Chicago Medical School.This study was supported in part by Grant No. P217 from the American Cancer Society.Requests for reprints should be addressed to Paul Heller, M.D., Veterans Administration West Side Hospital, 820 South Damen Avenue, Chicago 12, Illinois. 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GRODE, M.D., JOHN LASZLO, M.D.Serum Lactic Dehydrogenase Activity and Isoenzyme Patterns in Some Haematological DiseasesZur vergleichenden Bestimmung von Fermenten im Sternalmark- und Venenblutserum bei inneren KrankheitenThe Activity of Lactic Dehydrogenase in Megaloblastic AnaemiaFermentuntersuchungen bei experimenteller bleivergiftungDie Erh�hung der Glucose-6-Phosphatdehydrogenaseaktivit�t der Erythrocyten und ihre klinische BeurteilungÜber die Bedeutung der Glukose-6-Phosphat-Dehydrogenase-Aktivität in Erythrozyten für die Differentialdiagnose der AnämienÜber die Bedeutung der Glukose-6-Phosphat-Dehydrogenase-Aktivität in Erythrozyten für die Differentialdiagnose der AnämienThalass�mie in DeutschlandExcessively high levels of lactic acid dehydrogenase activity in pernicious anemiaReferencesDehydrogenases: Glucose-6-Phosphate Dehydrogenase, 6-Phosphogluconate Dehydrogenase, Glutathione Reductase, Methemoglobin Reductase, Polyol DehydrogenasesLactic dehydrogenase activity in gastric juice for the diagnosis of gastric cancerA comparative study of enzymic activities in normal adult and cord blood erythrocytes as related to the reduction of methemoglobinProblem3s in the Differentiation of the Megaloblastic Anemias 1 November 1960Volume 53, Issue 5Page: 898-913KeywordsAnemiaBlood plasmaChartsDehydrogenasesEnzymesHospital medicineMedical servicesMegaloblastic anemiaPlasma cellsResearch laboratories ePublished: 1 December 2008 Issue Published: 1 November 1960 PDF downloadLoading ...