Cyclic nucleotides on cell growth in vitro.

Abstract

Summary The growth of four tumorigenic cell lines was inhibited from 70 to 89% by adenosine 3′, 5′-cyclic monophosphate (0.3 mm) whereas a nonmalignant cell line (WI-38) was affected only slightly (13%). Adenosine 2′,3′-cyclic monophosphate (0.3 mm) was less active, inhibiting the tumorigenic cell lines from 23 to 56%. The other cyclic purine nucleotides investigated (guanosine 3′,5′-cyclic monophosphate and quanosine 2′,3′-cyclic monophosphate) did not show selectivity; they inhibited the tumorigenic strains and the WI-38 cell line. The 3′,5′-cyclic pyrimidine nucleotides, with the exception of thymidine 3′,5′-cyclic monophosphate, did not significantly inhibit any of the cell lines. Thymidine 3′,5′-cyclic monophosphate produced inhibition of only strain L.