Time to knit · 2000 · 35 citations · 0 references
Life SciencesBiotechnologyMicrobiologyBiochemical EngineeringBioprocess EngineeringIndustrial BiotechnologyBacterial FermentationFermentation KineticsFermentation MicrobiologyMicrobial FermentationMicrobial Fermentation BioreactorsAerationAeration StrategiesAgitationBiodegradable FeedstocksCell Mass AccumulationPolyhydroxyalkanoate ProductionPolyhydroxyalkanoatesTransfer Coefficient
Production of biodegradable plastic, polyhydroxyalkanoate (PHA) was demonstrated using culture of moderately haloakalitolerant Halomonas campisalis MCMB-1027. Production of PHA was carried out at different aeration (0.5-1, 1, and 1.5 vvm) and agitation (100,150 and 200 rpm) in 14 L fermenter. Maximum production of PHA was 40.69% on the basis of dry cell mass at 1 vvm and 100 rpm. Material balance over maltose revealed that aeration 1 vvm and agitation 100 rpm was optimum for conversion of utiliz