AN ELECTROPHORETIC STUDY OF PLASMA AND PLASMA FRACTIONS OF NORMAL AND INJURED RATS

Abstract

The development of the electrophoretic procedure has led a number of in%-estigators to study the distribution of rat plasma proteins by this method. Rat serum or plasma does not yield the distinct pat,terns that are obtained with human plasma. Moore, Levin, and Leathem (1) could not observe the cr-globulin fraction in normal rat serum dialyzed against phosphate buffer at pH 7.4. After hypophysectomy (1) and thyroidectomy or thiouracil feeding in rats ( When normal rat serum was examined at pH 8.5, the a-globulin component was seen. Deutsch and Goodloe (3) found no distinct component in the region of t,he a-globulin at pH 8.6 Although five components have been described (1, 4), the boundaries are too indistinct for accurate analysis.