The Effect of Kaolin on Immunoglobulins: An Improved Technique to Remove the Nonspecific Serum Inhibitor of Reovirus Hemagglutination

Abstract

Summary The present work has shown that removal of nonspecific inhibitors of hemagglutination by kaolin resulted in the loss of 40 to 60% of the immunoglobulins. The nonspecific inhibitor of reovirus hemagglutination has been shown to be either β-lipoprotein or a substance associated with β-lipoprotein. It was also shown that this inhibitor could be effectively removed by the heparin-MnCl2 method. This method was found to give higher HI antibody titer than kaolin and to remove less than 10% of the immunoglobulins.