Journal of Virology · 1968 · 21 citations · 15 references
Life SciencesBiologyMolecular BiologyMolecular VirologyRna BiologyVirologyOncogenic VirusesRibonucleic Acid (Rna) ChemistryViral MaturationViral Rna BiologyVirus PurificationRibonuclease ChemistryRibonucleic Acid ExtractionRna ExtractionRna Tumor VirusesSedimentation Coefficient AnalysisViral Nucleic Acid ExtractionViral Rna Synthesis
Virus particles were continuously produced by a cell line (78A1) of rat embryo fibroblasts that had been transformed by the murine sarcoma-leukemia virus complex. Since most of the mature virions were found in the extracellular fluid and were not cell-associated, a measurable quantity of viral ribonucleic acid (RNA) could not be extracted from these cells. Cycloheximide, a protein inhibitor, was successfully used to accumulate viral RNA within the cells. This ribonuclease-sensitive RNA, with a sedimentation coefficient of 71S, had the same base composition as the high molecular weight RNA (S(20,w) = 71) isolated from purified virions released by the transformed cells.
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