Two populations of Ia-like molecules on a human B cell line.

TLDR

The study reports two distinct Ia‑like molecule populations on the human B cell line Raji. Two monoclonal antibodies, L203 and L227, were used to immunoprecipitate 34,000‑ and 28,000‑dalton Ia‑like proteins from 125I‑labeled Raji extracts, with L227 also pulling down a 25,000‑dalton species. Immunodepletion experiments confirmed that the antibodies target two distinct Ia‑like molecule populations.

Abstract

Abstract Evidence is presented for two populations of Ia-like molecules on the human B cell line Raji. Two monoclonal antibodies, L203 and L227, are described. Both recognize nonpolymorphic determinants on B cell antigens. Both immunoprecipitate 34,000- and 28,000-dalton material from 125I-labeled extracts of Raji, but L227 precipitates 25,000-dalton material as well. Immunodepletion experiments confirm that the antibodies react with two different populations of molecules.