The Journal of Immunology · 1980 · 1.1K citations · 0 references
Life SciencesImmunologyMolecular BiologyCellular ImmunologyMolecular ImmunologyMonoclonal AntibodiesAntibody CharacterizationB Cell ActivationB Cell BiologyHumoral ImmunityB Cell Antigen RecognitionB-cell EpitopesB-cell Immunophenotypes
The study reports two distinct Ia‑like molecule populations on the human B cell line Raji. Two monoclonal antibodies, L203 and L227, were used to immunoprecipitate 34,000‑ and 28,000‑dalton Ia‑like proteins from 125I‑labeled Raji extracts, with L227 also pulling down a 25,000‑dalton species. Immunodepletion experiments confirmed that the antibodies target two distinct Ia‑like molecule populations.
Abstract Evidence is presented for two populations of Ia-like molecules on the human B cell line Raji. Two monoclonal antibodies, L203 and L227, are described. Both recognize nonpolymorphic determinants on B cell antigens. Both immunoprecipitate 34,000- and 28,000-dalton material from 125I-labeled extracts of Raji, but L227 precipitates 25,000-dalton material as well. Immunodepletion experiments confirm that the antibodies react with two different populations of molecules.