Natural Product Communications · 2009 · 52 citations · 11 references
Life SciencesMicrobiologyPlant PathologyMass Spectrometric AnalysisMycologyPlant BiochemistryAntifungal AgentsMedicinal PlantsBotanical BioassaysCrude Extract PreparationExtractIsoquinoline AlkaloidsMinimum Inhibitory Concentration DeterminationMinimum Inhibitory Concentration TestingNatural Antifungal CompoundsPlant Alkaloids
Bioassay-guided fractionation of the crude extract of the whole plant of Macleaya cordata R. Br. led to the isolation of four alkaloids, which were identified as sanguinarine (1), chelerythrine (2), protopine (3) and alpha-allocryptopine (4) on the basis of their physicochemical and spectrometric data. Compounds 1 and 2 demonstrated a significant antifungal activity against the six test fungi with median inhibitory concentrations (IC50) ranging from 0.47 to 6.13 microg/mL. Compound 1 was the most effective with an IC50 of 0.47 microg/mL on Rhizoctonia solani. Furthermore, compounds 1 and 2 also demonstrated strong antibacterial activity, with IC50 values ranging from 5.01 to 11.3 microg/mL, and minimum inhibitory concentrations (MIC) ranging from 8.0 to 32.0 microg/mL. This is the first report on the activity of the alkaloids from M. cordata against economically important phytopathogenic fungi and bacteria.
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Comparison of alamar blue and MTT assays for high through-put screening
Roslida Abd Hamid, Yakov Rotshteyn, Leyana Rabadi et al. · Toxicology in Vitro · 2004 · 568 citations