Genetic analysis of several root hair mutants of barley

Abstract

The mutagenic treatment of barley varieties ‘Rudzik’, ‘Dema’, ‘Krona’, ‘Diva’, ‘Karat’, and ‘Delisa’ was performed with the use of N-methylo-N-nitroso-urea (MNU) and sodium azide. To generate high frequency of gene mutations, the combined treatment with both mutagenes (Szarejko and Maluszynski, 1999) or double treatment of MNU was applied. The doses for treatment of Rudzik and Dema varieties were as follows: 1.5 mM NaN3/3h – inter-incubation germination/6h – 0.75 mM MNU/3h and for variety Krona: 1.75 mM NaN3/3h – inter-incubation germination/6h – 0.9 mM MNU/3h. For the varieties Diva, Karat and Delisa double treatment of MNU was used: 0.7 mM MNH/3h. - inter-incubation germination/6h - 0.7 mM MNH/3h. The selection of root hair mutants was performed in the M2 or M3 generations. Subsequent generations (M4-M6) were used to perform homozygosity test and multiply seeds of homozygous lines with mutated root hair phenotype. The observations of root hair zone were done for plants growing in aeroponic conditions (Fig.1). The inheritance of the root hair phenotypes was analyzed in the F1 and F2 generations from the crosses between mutants and their parent varieties. Allelism test between mutants was performed. Results