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A Mutant of Hog Cholera Virus Inducing Interference in Swine Testicle Cell Cultures
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1970
Year
Diagnostic VirologyViral ReplicationMolecular VirologyPathogenesisImmunologyViral PathogenesisVirologyHog CholeraHc VirusPorcine DiseaseSwine VirusClonal PreparationsMicrobiologyReproductive BiologyMedicineViral ImmunityAnimal Virus
SUMMARY Two clonal preparations designated E + and E - hog cholera ( hc ) viruses, which differed in interactions with Newcastle disease ( nd ) viruses in swine testicle ( st ) cell cultures, were obtained during adaptation of hc virus to guinea pig kidney cells. The E + virus exerted an enhancing effect on the cytopathic effect ( cpe ) of nd virus in st cells, whereas the E - virus inhibited multiplication of nd virus in st cells. After 4 passages at limiting dilution, E - virus retained its distinctive character against nd virus. A quantitative assay procedure was developed for E - virus, using its interfering action. Although attenuated and wild strains of viruses were antigenically similar, attenuated virus was more sensitive to specific antibody than was the wild viral strain.